Human peripheral B cell development
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Typ
Examensarbete för masterexamen
Master Thesis
Master Thesis
Program
Publicerad
2011
Författare
Holmqvist, Joel
Modellbyggare
Tidskriftstitel
ISSN
Volymtitel
Utgivare
Sammanfattning
Since the middle of the nineties, our understanding of human peripheral B cell development has gradually increased. Today, the B cell compartment has been divided into three main populations; transitional, naive and memory B cells. In Mats Bermark's laboratorium, a cell surface marker identifying a novel peripheral B cell population has been found. The work performed during this thesis aimed to characterize the novel B cell population by comparing it with the major already described peripheral B cell populations. Novel B cells were found to be smaller than memory B cells. The antibody genes were found to contain less mutations than those of memory B cells. Furthermore, novel B cells were found to be less prone to enter apoptosis than transitional B cells. A screening experiment using flow cytometry added support to the hypothesis that the novel B cells are different from the already described populations. The results suggests that the novel B cell population is a distinct subpopulation post- or parallel to the naive population. Novel B cells might be able to differentiate into IgM+ memory B cells. In order to verify this, new experiments are needed
Beskrivning
Ämne/nyckelord
Energi , Livsvetenskaper , Industriell bioteknik , Energy , Life Science , Industrial Biotechnology