Screening potential lignin-degrading microorganisms and evaluating their optimal enzyme producing growth conditions
Ladda ner
Typ
Examensarbete för masterexamen
Master Thesis
Master Thesis
Program
Publicerad
2011
Författare
Swe, Kyi Tha
Modellbyggare
Tidskriftstitel
ISSN
Volymtitel
Utgivare
Sammanfattning
Wood or lignocellulosic biomass is a low cost and abundant resource that can be used in the large scale production of fuels and chemicals. The presence of covalent lignin-carbohydrate linkages between sugar hydroxyl of hemicelluloses and phenylpropane subunits in lignin gives lignocellulose protection against degradation. Separation and modification of the lignocellulosic materials are required in order for it be used, where microbial modification of the biomass is one alternative. The aim of this project is to identify strains of filamentous fungi with high potential in the degradation of lignocellulose compounds. Birch wood and wheat bran were used as substrate for the induction of lignocelluloses degrading enzymes production in fungi. Seven strains of filamentous fungi from the Industrial Biotechnology group at Chalmers University of Technology were chosen and they were screen for the ability to grow on birch containing agar medium and decolorization of synthetic dye, RBBR, methyl green and guaiacol as an indicator for the production of lignin degrading enzymes. Among seven strains of fungi, Penicillium pinophilum (IBT 10872) and Trametes hirsuta (NCIM 1201) were chosen for cultivation in different liquid media and solid state culture (SSC) and the amount of enzyme produced were analysed. Both P. pinophilum and T. hirsuta were found to produce higher amount of cellulase and xylanase enzymes when they were grown in birch wood liquid medium containing peptone compare to medium without peptone. Moreover, solid state culture of T. hirsuta on wheat bran was also found to induce the lignin degrading enzyme, laccase but no manganese peroxidase activity was detected.
Beskrivning
Ämne/nyckelord
Livsvetenskaper , Mikrobiologi , Industriell bioteknik , Life Science , Microbiology , Industrial Biotechnology