Etanolbildande acetogener för syngasfermentering –en jämförande studie

Typ
Examensarbete för kandidatexamen
Bachelor Thesis
Program
Bioteknik 300 hp (civilingenjör)
Publicerad
2018
Författare
Mårtenson, Sara
Stavås, Emma
Hadi, Fahim
Landström, Carl-Johan
Frithiofson, Emil
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The use of fossil fuels contributes to global warming, which is one of the biggest pro-blems in today’s society. An alternative to reduce the use of fossil fuels is to instead use renewable fuels, such as bioethanol. One way to produce bioethanol is to allow anaerobic microorganisms to ferment syngas, a mixture of carbon monoxide, carbon dioxide and hydrogen gas. The purpose of the work was to evaluate and rank microorganisms, which by fermentation of syngas, have the greatest potential for high ethanol production. A literature review gathered information of around 100 microorganisms to find the orga-nisms with the greatest potential for ethanol production. The results showed that Clostri-dium autoethanogenum, Clostridium carboxidivorans, Clostridium coskatii, Clostridium ljungdahlii and Clostridium ragsdalei were the microorganisms with the greatest poten-tial. In addition to the literature study, an experiment was performed where inhibitor tolerance was examined. The inhibitor ammonia was added to C. ljungdahlii, C. autoethanogenum and C. carboxidivorans. These three organisms were deemed to have high potential in the literature review and were available at the institution. The result showed that no organism grew at 100 mM of ammonia. C. ljungdahlii showed the highest tolerance as neither ethanol formation nor growth decreased significantly at 50 mM and below. The ethanol production of C. autoethanogenum decreased already at 35 mM. The ethanol production C. carboxidivorans decreased at 50 mM ammonia. The work was carried out at Chalmers University of Technology.
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Ämne/nyckelord
Livsvetenskaper , Kemiska processer , Bioenergi , Förnyelsebar bioenergi , Life Science , Chemical Process Engineering , Bioenergy , Renewable Bioenergy Research
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