Encapsulation and Controlled Release of Benzalkonium Chloride - Production of Antimicrobial Cellulose Nonwoven Fabrics for Wound Treatment
Typ
Examensarbete för masterexamen
Program
Materials chemistry (MPMCN), MSc
Publicerad
2021
Författare
Karlsson, Jakob
Modellbyggare
Tidskriftstitel
ISSN
Volymtitel
Utgivare
Sammanfattning
Encapsulation and controlled release of an active substance is sought-after due to
its many applications, were one of them is chronic wound treatment. By having a
continuous release of the antimicrobial agent the risk of infection is reduced and
the lifetime of the wound dressing will be increased. The aim of this project
is to encapsulate Benzyldimethylhexadecylammonium chloride (BAC) into polymeric
microspheres to obtain a controlled release rate and then incorporate the
microspheres into cellulose nonwoven fabrics. The microspheres are formulated
using the internal phase separation via solvent evaporation methodology. Microscopic
techniques are used to evaluate size and morphology of the microspheres.
The encapsulation efficiency and release rate from the microspheres are evaluated
both from microspheres suspensions and microspheres incorporated into fibres using
UV-Vis spectroscopy and LC-MS.
Based on microscopic imagery does poly(D,L-lactic-co-glycolic acid) (PLGA) formulate
microspheres of desired size, morphology and encapsulation efficiency. By
adding NaCl to the continuous phase, encapsulation efficiency of BAC was increased
along with an increased microsphere size, however long term stability of
the microspheres was reduced. Due to BAC’s poor solubility in water containing
NaCl, does crystallisation of BAC occur in the continuous phase. Other factors
effecting the encapsulation efficiency of BAC was type of solvent used, evaporation
method and the amount of BAC added during formulation. Furthermore, incorporation
of microspheres into cellulose nonwoven fabrics were successful, and the
particles was well dispersed in the fibre.
The release of BAC from polymeric microspheres was slow, and it takes approximately
100 hours until a significant release can be seen. This was observed for
release studies from both microsphere suspensions and microspheres incorporated
into cellulose fabrics. The conclusion is then that a controlled and reduced release of
BAC via microencapsulation can be obtained due to the polymeric material acting
as a diffusion barrier.
Beskrivning
Ämne/nyckelord
microsphere , internal phase separation method , controlled release , poly(d,llactic- co-glycolic acid) , cellulose nonwovens by solution blowing