Examensarbeten för kandidatexamen // Bachelor Theseshttp://hdl.handle.net/20.500.12380/422024-03-28T13:58:05Z2024-03-28T13:58:05Z991A study of electrical treeing in XLPE using wire electrodes - Impact on electrical treeing inception voltage by sample preparation and voltage stabilizersKronlund, LinusKvist, Patrichttp://hdl.handle.net/20.500.12380/1426742019-07-03T12:35:41Z2011-01-01T00:00:00Zdc.title: A study of electrical treeing in XLPE using wire electrodes - Impact on electrical treeing inception voltage by sample preparation and voltage stabilizers
dc.contributor.author: Kronlund, Linus; Kvist, Patric
2011-01-01T00:00:00ZAlkylering av etengas över zeolit H-betakatalysator med silica sol som bindemedelBelanovic, DaliborHulting, FredrikLinder, KristinaMalmström, EmilRazavi, SamanUzelac, Vanjahttp://hdl.handle.net/20.500.12380/1610122023-02-09T16:42:01Z2012-01-01T00:00:00Zdc.title: Alkylering av etengas över zeolit H-betakatalysator med silica sol som bindemedel
dc.contributor.author: Belanovic, Dalibor; Hulting, Fredrik; Linder, Kristina; Malmström, Emil; Razavi, Saman; Uzelac, Vanja
2012-01-01T00:00:00ZAminering och alkylering av glycerol via Hydrogen AutotransferRönnberg, LouiseJonsson, JuliaLarsson, AdamOlsson, JoakimLundström, HelenaNilsson, Victorhttp://hdl.handle.net/20.500.12380/2197952023-02-09T16:41:03Z2015-01-01T00:00:00Zdc.title: Aminering och alkylering av glycerol via Hydrogen Autotransfer
dc.contributor.author: Rönnberg, Louise; Jonsson, Julia; Larsson, Adam; Olsson, Joakim; Lundström, Helena; Nilsson, Victor
2015-01-01T00:00:00ZAmylasers enzymatiska aktivitet i saltvattensmiljöVimpari, Silvanahttp://hdl.handle.net/20.500.12380/1377652019-07-03T12:32:32Z2011-01-01T00:00:00Zdc.title: Amylasers enzymatiska aktivitet i saltvattensmiljö
dc.contributor.author: Vimpari, Silvana
dc.description.abstract: Amylases are enzymes that digest starch by cleavage of glycosidic bonds, therby releasing glucose, maltose and/or malto-oligosaccharides. Amylase is used for biotechnological purposes such as the production of biofuels, in the food industry where starch is found naturally in cereals, but also in the paper industry and in the manufacture of detergents. The difference between α-amylase, β-amylase and glucoamylase is how they cleave starch into smaller glucose-containing units. α-amylase breaks down starch into oligosaccharides and dextrins, while β-amylase breaks down starch into maltose and amyloglucosidase to glucose. The aim of the project was to investigate how the enzyme activity of industrial purified amylases, that are commercially available, are affected by saline environments compared to freshwater conditions. Another part of the project was to isolate marine bacteria that use starch as a carbon source and thus produce amylases. This was performed by isolation and purification of the bacteria in starch medium, characterization in shake flask cultures and measurement of enzyme activity. Two strains were found that had better cell growth, released larger amounts of glucose from the starch, and showed a higher enzymatic activity than the other in seawater. The industrially produced amylases were tested for dependence on various parameters such as pH, temperature and enzyme activity. α-amylase Aspergillus oryzae, β-amylase and amyloglucosidases from Aspergillus niger were activated at pH 4.8. α-amylases Bacillus subtilis and porcine were activated at pH 6.9. Enzyme activity measurments for the industrially isolated amylases showed that a number of those tested had a higher amylase activity in seawater than in freshwater whereas the α-amylases from Bacillus licheniformis and Aspergillus oryzae did not exhibit any activity in seawater. This may be due to the substances found in seawater helps to facilitate the binding of and reaction with substrates and/or stabilizes the protein structure of a beneficial form. A possible reason for the differences observed between similar amylases may be that different types of treatment processes at the manufacturing companies can provide a finished product that has a different sensitivity to ions. To use algae as a future fuel source and cultivate them in saltwater you need to have a salt-tolerant amylase that can break down large amounts of starch. More analyses of existing purified amylases is recommended as these show that there is potential to find amylases that acutally are activated better in saltwater than freshwater.
2011-01-01T00:00:00ZAmyloidbildning av Parkinsonproteinet -synuklein i cell-liknande miljö, in vitro, med crowding agents dextran och PEGLöfgren, EmilPernhed, DavidPernsved, MonicaPersson, ElinRehnberg, NikitaSvensson, Ceciliahttp://hdl.handle.net/20.500.12380/3026852023-02-09T16:41:20Z2021-01-01T00:00:00Zdc.title: Amyloidbildning av Parkinsonproteinet -synuklein i cell-liknande miljö, in vitro, med crowding agents dextran och PEG
dc.contributor.author: Löfgren, Emil; Pernhed, David; Pernsved, Monica; Persson, Elin; Rehnberg, Nikita; Svensson, Cecilia
dc.description.abstract: All over the world life expectancy is increasing which leads to a higher prevalence
of neurodegenerative diseases such as Parkinson’s Disease. The disease is chronic and
leads to slow degradation of cells in the nervous system of the brain. -synuclein is a
well-studied protein which is likely involved in Parkinson’s Disease.
The purpose of this project is to experimentally study the aggregation kinetics of -
synuclein in regard to excluded volume effects. Different sizes and concentrations of two
macromolecular crowding agents, dextran and PEG, are used to mimic the crowded
cell environment. Crowded conditions and their effect on the formation of -synuclein
amyloids and aggregetion kinetics, are studied through a Thioflavin T-aggregation assay.
SDS-PAGE, circular dichroism spectroscopy and AFM are used to complement and
validate the assay results.
Higher concentrations of crowding agents generates faster aggregation of -synuclein
compared to lower concentrations. However the aggregation does not always increase
linearly with crowding. Certain crowding agents promote elongation while others
promote alternative mechanisms such as primary or secondary nucleation. Several experiments
verify the occurance of soft interactions between PEG and -synuclein. The
-synuclein aggregation process is rather complex and dependent on several factors.
Crowding promotes faster aggregation and morphology studies suggest the formation
of amyloid fibrills. The results verify the effect of excluded volume but further research
is required to determine which part of the aggregation process it primarily affects.
2021-01-01T00:00:00ZAnalys av slam från PWR-ånggeneratorerEwertsson, Mathildehttp://hdl.handle.net/20.500.12380/3026922023-02-09T16:40:46Z2021-01-01T00:00:00Zdc.title: Analys av slam från PWR-ånggeneratorer
dc.contributor.author: Ewertsson, Mathilde
2021-01-01T00:00:00ZBildande av kol-kvävebindningar genom metallkatalyserade reaktionerBylund, JenniferDamgaard, CarolineLarsson, MikaelLuong, StaffanMelkersson, JennyÖjerborn, Johanhttp://hdl.handle.net/20.500.12380/2001392023-02-09T16:41:46Z2014-01-01T00:00:00Zdc.title: Bildande av kol-kvävebindningar genom metallkatalyserade reaktioner
dc.contributor.author: Bylund, Jennifer; Damgaard, Caroline; Larsson, Mikael; Luong, Staffan; Melkersson, Jenny; Öjerborn, Johan
2014-01-01T00:00:00ZBiofysikaliska studier av amyloida proteinfibrillers kompakteringBengtsson, MatildeNassif, JessyRundberg, LisaSchmidt, EneasSundin, ElinSvensson, Emeliehttp://hdl.handle.net/20.500.12380/2001422023-02-09T16:41:24Z2014-01-01T00:00:00Zdc.title: Biofysikaliska studier av amyloida proteinfibrillers kompaktering
dc.contributor.author: Bengtsson, Matilde; Nassif, Jessy; Rundberg, Lisa; Schmidt, Eneas; Sundin, Elin; Svensson, Emelie
2014-01-01T00:00:00ZCatalyzed production of alkenyl succinic anhydrideEnglund, Mattiashttp://hdl.handle.net/20.500.12380/1533712019-07-03T12:46:11Z2011-01-01T00:00:00Zdc.title: Catalyzed production of alkenyl succinic anhydride
dc.contributor.author: Englund, Mattias
2011-01-01T00:00:00ZCementation as a means for leachate liquor purificationSöderqvist, FridaLarsson, AntonLjungberg, DavidAtterfelt, JoakimGustafsson, Gabrielhttp://hdl.handle.net/20.500.12380/2197892023-02-09T16:41:42Z2015-01-01T00:00:00Zdc.title: Cementation as a means for leachate liquor purification
dc.contributor.author: Söderqvist, Frida; Larsson, Anton; Ljungberg, David; Atterfelt, Joakim; Gustafsson, Gabriel
2015-01-01T00:00:00Z