Targeted microbial ensilage additives for Laminaria digitata and Saccharina latissima

Examensarbete för masterexamen

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Type: Examensarbete för masterexamen
Master Thesis
Title: Targeted microbial ensilage additives for Laminaria digitata and Saccharina latissima
Authors: Malmhäll-Bah, Eric
Abstract: Seaweeds are a potential feedstock for biofuel and biochemical production. However, seaweed biomass is subject to rapid degradation. This project aims at evaluating the prospect of using microbial additives in an ensiling process for the preservation of the brown seaweed Laminaria digitata and Saccharina latissima. Lactic acid bacteria (LAB) isolated from seaweed silage may be suitable as microbial ensilage additives as they thrive in that type of environment. Seven bacterial strains were isolated from seaweed silage of the species L. digitata, and were determined to belong to the genus Lactobacillus. The bacterial isolates were screened for growth on the carbohydrates laminarin and mannitol, which are storage carbohydrates in the kelp species of interest. The results from the screening showed that the isolates grew on mannitol but not on laminarin. Two strains were selected for further investigation of products formed during growth on mannitol. The main products formed for both strains were lactate and acetate. In continuation, these strains were tested as microbial ensilage additives for S. latissima and L. digitata. The effectiveness of the strains as ensilage additives was compared to a chemical additive and ensiling without additives. Biomass retention was monitored over the course of the ensiling assuming biomass loss as gases was due to unwanted fermentation. The isolates had a positive effect retaining 1 % more biomass, compared to ensiling without any additives. However, there was no significant difference between the two stains. Furthermore, the isolates were slightly outperformed by the chemical additive.
Keywords: Livsvetenskaper;Biologiska vetenskaper;Industriell bioteknik;Life Science;Biological Sciences;Industrial Biotechnology
Issue Date: 2016
Publisher: Chalmers tekniska högskola / Institutionen för biologi och bioteknik
Chalmers University of Technology / Department of Biology and Biological Engineering
Collection:Examensarbeten för masterexamen // Master Theses

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