Tackling Metastatic Cancer: From Systems Biology to Therapeutics
Typ
Examensarbete för masterexamen
Program
Publicerad
2019
Författare
Limeta, Angelo
Modellbyggare
Tidskriftstitel
ISSN
Volymtitel
Utgivare
Sammanfattning
Most cancer associated deaths are due to metastasis, the process in which a primary tumor
migrates into neighboring tissues and forms secondary metastases. This project is split into
two related parts, systems biology and treatment design for metastatic cancer. Systems
biology approaches to cancer require large biological data sets, such as genomic and
transcriptomic profiling of tumors. Previous data gathering efforts such as The Cancer
Genome Atlas (TCGA) have mainly consisted of primary tumor samples, which makes the
metastatic process still poorly characterized to this date. Although a recent study by Robinson
et al. have performed RNA-seq on a wide array of metastases, coined the MET500 cohort.
Differential gene expression analysis between primary TCGA tumor samples and metastatic
MET500 tumor samples revealed a potential mechanism for colonization of a secondary site,
by utilizing the Mesenchymal-Epithelial Transition (MET). The treatment design part of this
project sought to implement a novel CRISPR-Cas13 base editing treatment against a recently
characterized mechanism for breast cancer metastasis.
Beskrivning
Ämne/nyckelord
Cancer , Metastasis , RNA-seq , Mesenchymal-to-Epithelial Transition , CRISPRCas13, , base editing