Polyelectrolyte brushes on porous carbon electrodes for protein capture and electrochemical release
| dc.contributor.author | Bång, Elin | |
| dc.contributor.department | Chalmers tekniska högskola / Institutionen för kemi och kemiteknik | sv |
| dc.contributor.department | Chalmers University of Technology / Department of Chemistry and Chemical Engineering | en |
| dc.contributor.examiner | Dahlin, Andreas | |
| dc.contributor.supervisor | Ferrand-Drake del Castillo, Gustav | |
| dc.contributor.supervisor | Olsson, Oliver | |
| dc.date.accessioned | 2024-10-22T13:48:33Z | |
| dc.date.available | 2024-10-22T13:48:33Z | |
| dc.date.issued | 2024 | |
| dc.date.submitted | ||
| dc.description.abstract | Biopharmaceuticals are medical drugs produced from biological components. Necessary steps in the manufacturing includes isolation and purification of biomolecules, for which chromatography is a common technique. However, it requires harmful elution chemicals, that can affect the desired product. To achieve a more efficient and non-invasive way for biopharmaceutical production, there is an interest of developing new methods for purification of biomolecules. The electrode surface of Nyctea Technologies utilizes polyelectrolyte brushes responsive to pH-stimuli. By alternating pH, or by electric signals, the brushes can switch between charged and neutral state. This enables interactions that allows the brushes to reversible capture and release biomolecules like proteins. The polymer coating has previous been applied to gold coated steel surfaces. The aim of this project was to investigate alternative substrate materials with the purpose to improve the protein binding capacity of the electrodes. Displaying attractive properties such as high chemical stability and electrical conductivity, having high specific surface area, and high availability at low cost, porous carbon electrode materials was further explored. The synthesis of poly(methacrylic acid) (PMAA) brushes was performed on reticulated vitreous carbon (RVC) and on carbon felt. By thermogravimetric analysis (TGA) the polymer amount on the coated materials could be quantified. It was confirmed to be PMAA on the felt, while the RVC showed no clear signs of attached polymer. The protein binding capacity of the carbon electrodes was evaluated by pH controlled catch and release of protein, using bovine serum albumin (BSA). The RVC was unsuccessful while the felt preformed better, and was chosen to be further developed as an electrode material. The carbon felt electrodes was assembled in capsules and connected to a chromatography system. Results made clear that protein was captured by the electrodes, and protein release was possible both by pH elution and by electrochemistry, making the carbon felt a promising candidate for future research. | |
| dc.identifier.coursecode | KBTX12 | |
| dc.identifier.uri | http://hdl.handle.net/20.500.12380/308939 | |
| dc.language.iso | eng | |
| dc.setspec.uppsok | PhysicsChemistryMaths | |
| dc.subject | Polyelectrolyte brushes | |
| dc.subject | Porous carbon | |
| dc.subject | Protein immobilization | |
| dc.subject | Electrochemistry | |
| dc.title | Polyelectrolyte brushes on porous carbon electrodes for protein capture and electrochemical release | |
| dc.type.degree | Examensarbete för masterexamen | sv |
| dc.type.degree | Master's Thesis | en |
| dc.type.uppsok | H | |
| local.programme | Materials chemistry (MPMCN), MSc |