The effect of sample composition acoustic droplet ejection of small molecules in DMSO
dc.contributor.author | Ladisic, Sebastian | |
dc.contributor.department | Chalmers tekniska högskola / Institutionen för kemi och kemiteknik | sv |
dc.contributor.department | Chalmers University of Technology / Department of Chemistry and Chemical Engineering | en |
dc.contributor.examiner | Larsson, Anette | |
dc.contributor.supervisor | Svensson, Martin | |
dc.date.accessioned | 2023-07-04T12:42:22Z | |
dc.date.available | 2023-07-04T12:42:22Z | |
dc.date.issued | 2023 | |
dc.date.submitted | 2023 | |
dc.description.abstract | AstraZeneca is pioneering within life science research and development, and contributes significantly in reducing the numbers of deaths caused by non-communicable diseases. Laboratory automation has contributed to the heavily increased number of screening assays in modern times and AstraZeneca has teamed up with Brooks Life Sciences, Beckman Coulter Life Sciences and Titian, to create a workflow that uses acoustic droplet ejection for transferring low volumes of liquid samples in an efficient and risk free process. Samples that pose problems in the liquid handler are removed from the chemical library and thus slow down the drug discovery and development process. This study evaluates any correlation between failure in acoustic dispensing and the sample composition of 50 suspensions of different small molecules in dimethyl sulfoxide. The samples are characterized by their solid particle shape, size and crystallinity, and a liquid chromatography - mass spectrometry method is developed to determine if the precipitate represents the original compound. Five sizes of poly(methyl metacrylate) microspheres are used in model suspensions for evaluating the interaction between solid particles and the acoustic signals. The results show that no suspension is contaminated and that the acoustic dispensing is not dependent on size, shape or crystallinity of the precipitate particles. The dispensing failure probably occurs from a combination of reflection and scattering of the acoustic signal that measures the sample volume in the tubes, and seems to be dependent on the amount of solid particles and their position on the tube bottom. This study concludes that the bare presence of solid particles is enough to make a sample incompatible with acoustic dispensing, and a reasonable solution is to implement a separate workflow for compounds that are poorly soluble in the solvent of choice. An alternative workflow is presented in the final section of the report. | |
dc.identifier.coursecode | KBTX12 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12380/306571 | |
dc.language.iso | eng | |
dc.setspec.uppsok | PhysicsChemistryMaths | |
dc.subject | Acoustic dispensing | |
dc.subject | Sample characterization | |
dc.subject | Solubility | |
dc.subject | Liquid chromatography | |
dc.subject | Mass spectrometry | |
dc.title | The effect of sample composition acoustic droplet ejection of small molecules in DMSO | |
dc.type.degree | Examensarbete för masterexamen | sv |
dc.type.degree | Master's Thesis | en |
dc.type.uppsok | H | |
local.programme | Materials chemistry (MPMCN), MSc |
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