Long chain polyunsaturated fatty acids and allergy development Analysis of phospholipids in maternal and cord serum and their relationship with each other, diet and development of allergy
Examensarbete för masterexamen
Biotechnology (MPBIO), MSc
Allergy is an increasing problem today in the Western world. Even though the mechanism causing the allergic response is understood is it not known why some people develop allergy and some does not. The aim of the thesis was to investigate relation between long chain polyunsaturated fatty acids (LCPUFA) in the phospholipid fraction of maternal and cord serum and development of allergy. Phospholipids were extracted from maternal and cord serum using solid phase extraction columns. Composition of phospholipids was then analyzed using GC-MS. Wilcoxon signed rank test, Spearman’s correlation, Mann-Whitney U test and independent t-test was used to analyze the data. Relation between levels of fatty acids in mother and children were compared to each other as well to allergy at one year of age. Inﬂuence of diet on levels in mother and children as well as allergy was also examined. Levels of LCPUFA was found to correlate between mothers and children. Osbond acid and total n-6 PUFA was found in higher levels in children that developed eczema, whereas lower levels of docosahexaenoic acid (DHA) and total n-3 LCPUFA was found in mothers to children developing any allergy. Linoleic acid (LA) and n-6:n-3 PUFA ratio was also found to be higher in mothers to children developing any allergy. Daily intake of arachaidonic acid (AA) and DHA during the pregnancy was found to be lower in mothers to children who developed food allergy and asthma than in mothers to children who did not develop any allergy. No correlation between the diet and levels of fatty acid in mother and children were found. To conclude, a high proportion of n-6 PUFA in cord serum phospholipids and a low proportion of n-3 PUFA in maternal serum phospholipids was observed in relation to allergy and may inﬂuence the development of allergy.
LCPUFA, PUFA, allergy, cord serum, GC-MS, phospholipid