Characterization and differentiation of MAFA reporter hiPS cells into pancreatic beta cells

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dc.contributor.authorSjölin, Jacob
dc.contributor.departmentChalmers tekniska högskola / Institutionen för biologi och biotekniksv
dc.contributor.examinerEsbjörner Winters, Elin
dc.contributor.supervisorGupta, Shailesh
dc.date.accessioned2019-12-03T07:31:29Z
dc.date.available2019-12-03T07:31:29Z
dc.date.issued2019sv
dc.date.submitted2019
dc.description.abstractStem cell research is a wide field within science. One of the main research areas with the field is the differentiation of various cell types. However one of the main difficulties within this research area is to achieve a high yield of target cells from the differentiation process. This project aims at improving the yield of pancreatic beta cell differentiation by utilizing a reporter cell line for V-maf musculoaponeurotic fibrosarcoma oncogene homolog A (MAFA). This reporter line has genetically tagged MAFA with green fluorescent protein (GFP). As MAFA is only expressed in beta cells, this cell line can be used in order to sort beta cells, which are expressing the GFP-tagged MAFA, from non-beta cells using fluorescent activated cell sorting (FACS). The cells were differentiated from human pluripotent stem cells (hiPSCs) into beta cells and were characterized at different stages using flow cytometry and immunofluorescence. In this project six differentiation experiments were performed, showing that MAFA-GFP is successfully expressed by beta cells at the end stages of the differentiation process. However, additional research is required to perform the cell sorting and test to the insulin response to glucose of these cells.sv
dc.identifier.coursecodeBBTX03sv
dc.identifier.urihttps://hdl.handle.net/20.500.12380/300570
dc.language.isoengsv
dc.setspec.uppsokLifeEarthScience
dc.subjectimmunofluorescencesv
dc.subjectreporter linesv
dc.subjectinduced pluripotent stem cellssv
dc.subjectbeta cellssv
dc.subjectdifferentiationsv
dc.subjectflow cytometrysv
dc.subjectMAFAsv
dc.titleCharacterization and differentiation of MAFA reporter hiPS cells into pancreatic beta cellssv
dc.type.degreeExamensarbete för masterexamensv
dc.type.uppsokH
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