Evaluation of Small Molecules for Neuroectoderm differentiation & patterning using Factorial Experimental Design

dc.contributor.authorvoulgaris, dimitrios
dc.contributor.departmentChalmers tekniska högskola / Institutionen för fysik (Chalmers)sv
dc.contributor.departmentChalmers University of Technology / Department of Physics (Chalmers)en
dc.date.accessioned2019-07-03T14:25:04Z
dc.date.available2019-07-03T14:25:04Z
dc.date.issued2016
dc.description.abstractScreening for therapeutic compounds and treatments for diseases of the Brain does not only encompass the successful generation of iPS-derived homogenous neural stem cell populations but also the capacity of the differentiation protocol to derive on-demand region-specific cells. Νoggin, a human recombinant protein, has been extensively used in neural induction protocols but its high production costs and batch-to-batch variation have switched the focus to utilizing small molecules that can substitute noggin. Resultantly, the aim of this study was to optimize neuroepithelial stem cell generation in a cost-efficient fashion as well as to evaluate the impact that patterning factors (i.e. small molecules or proteins that enhance the emergence of type-specific neuronal populations) have on the regionality of the neural stem cell population. Findings in this study suggest that DMH1 is indeed a small molecule that can replace noggin in neural induction protocols as previously documented in literature; DMHI appears also to have a ventralizing effect on the generated neural population.
dc.identifier.urihttps://hdl.handle.net/20.500.12380/247469
dc.language.isoeng
dc.setspec.uppsokPhysicsChemistryMaths
dc.subjectMaterialvetenskap
dc.subjectGrundläggande vetenskaper
dc.subjectHållbar utveckling
dc.subjectInnovation och entreprenörskap (nyttiggörande)
dc.subjectMedicinteknik
dc.subjectMaterials Science
dc.subjectBasic Sciences
dc.subjectSustainable Development
dc.subjectInnovation & Entrepreneurship
dc.subjectMedical Engineering
dc.titleEvaluation of Small Molecules for Neuroectoderm differentiation & patterning using Factorial Experimental Design
dc.type.degreeExamensarbete för masterexamensv
dc.type.degreeMaster Thesisen
dc.type.uppsokH
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