Analysis of avenanthramides in oats with Ultra High Performance Liquid Chromatography

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dc.contributor.authorAstner, Martin
dc.contributor.departmentChalmers tekniska högskola / Institutionen för biologi och biotekniksv
dc.contributor.departmentChalmers University of Technology / Department of Biology and Biological Engineeringen
dc.date.accessioned2019-07-03T13:52:25Z
dc.date.available2019-07-03T13:52:25Z
dc.date.issued2016
dc.description.abstractAvenanthramides have been shown to have beneficial properties such as strong antioxidant activity and anti-inflammatory effects in humans, and are a key defence molecule for oats. The goal of this project was to create and validate a method for quantification of avenanthramides in oats that would take less than 20 minutes. A new extraction method and new liquid chromatography method were tested, as well as the introduction of an internal standard to improve quantification. We found that the internal standard selected was not stable during extraction and that it was necessary to use an external standard curve. We found that the liquid chromatography method was reproducible for standard compounds and that we could get separation of the three main avenathramides in oats for a total run time of 15 minutes. The extraction component of the method requires further work to improve recovery and stability before this method can be used for quantification of avenanthramides in oats.
dc.identifier.urihttps://hdl.handle.net/20.500.12380/232465
dc.language.isoeng
dc.setspec.uppsokLifeEarthScience
dc.subjectLivsvetenskaper
dc.subjectBiologiska vetenskaper
dc.subjectKemiteknik
dc.subjectLivsmedelsteknik
dc.subjectLivsmedelsvetenskap
dc.subjectLife Science
dc.subjectBiological Sciences
dc.subjectChemical Engineering
dc.subjectFood Engineering
dc.subjectFood Science
dc.titleAnalysis of avenanthramides in oats with Ultra High Performance Liquid Chromatography
dc.type.degreeExamensarbete på grundnivåsv
dc.type.uppsokM
local.programmeKemiteknik 180 hp (högskoleingenjör)
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