Development of a growth-sensitive malonyl-CoA platform strain

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dc.contributor.authorHedin, Alex
dc.contributor.departmentChalmers tekniska högskola / Institutionen för biologi och biotekniksv
dc.contributor.departmentChalmers University of Technology / Department of Biology and Biological Engineeringen
dc.date.accessioned2019-07-05T11:52:17Z
dc.date.available2019-07-05T11:52:17Z
dc.date.issued2019
dc.description.abstractThe transition from a fossil reliance society towards full reliance on sustainable alternatives is a difficult challenge. The development of high-performant microbial cell factories, which efficiency convert renewable carbon sources to generate industrially relevant compounds, are to date not economically compatible with the current less sustainable alternatives. Through metabolic reprogramming, the production of compounds at industrial levels could be obtained by increasing cofactors and substrates levels. In this thesis, we developed a growth-sensitive malonyl-CoA platform strain, able to select for an enrichment of the highly relevant precursor, malonyl-CoA. Through coupling it with a CRISPR-dCas9 gRNA library we performed a biosensor assisted laboratory evolution (BALE). Three targets, previously described to significantly increase the downstream product of malonylCoA, 3-Hydroxypropionic acid, were selected after 24 hours and more targets will be characterized.
dc.identifier.urihttps://hdl.handle.net/20.500.12380/256722
dc.language.isoeng
dc.setspec.uppsokLifeEarthScience
dc.subjectAnalytisk kemi
dc.subjectKemiska processer
dc.subjectEnergisystem
dc.subjectLivsvetenskaper
dc.subjectAnalytical Chemistry
dc.subjectChemical Process Engineering
dc.subjectEnergy Systems
dc.subjectLife Science
dc.titleDevelopment of a growth-sensitive malonyl-CoA platform strain
dc.type.degreeExamensarbete för masterexamensv
dc.type.degreeMaster Thesisen
dc.type.uppsokH
local.programmeBiotechnology (MPBIO), MSc
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