Hydrogels as scaffolds for stem cell therapy with focus on cartilage

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dc.contributor.authorForsell, Oliver
dc.contributor.departmentChalmers tekniska högskola / Institutionen för kemi och kemitekniksv
dc.contributor.departmentChalmers University of Technology / Department of Chemistry and Chemical Engineeringen
dc.contributor.examinerStröm, Anna
dc.contributor.supervisorStröm, Anna
dc.date.accessioned2023-07-03T07:41:27Z
dc.date.available2023-07-03T07:41:27Z
dc.date.issued2023
dc.date.submitted2023
dc.description.abstractOsteoarthritis, a disease arising from degradation of articular cartilage in joints, presents a major cost for the healthcare system and is a leading reason behind work disability. Conventional therapies are temporary fixes with limited applications and an inability to fully restore the damaged tissue. In recent years, cell therapy has emerged as an alternative and regenerative cartilage therapy. Challenges remain in developing proper scaffolds which serve the purpose of protecting the cells and preventing migration while providing the right mechanical and biochemical signals ensuring high cell viability, proper differentiation and phenotype maintenance. In this study, hydrogel scaffolds were formulated with the objective to achieve both sufficient biochemical and mechanical properties by attempting to create an interpenetrating network (IPN) of polymers forming physical and covalent crosslinks. To determine whether an IPN could be obtained, gels with a variety of crosslinking degrees and polymer concentrations were formulated using a Box-Behnken design of experiments. The gelation kinetics were investigated using a rheometer and molded gels were mechanically tested with a universal testing machine as well as structurally characterized by fluorescent microscopy. A positive synergistic effect was observed with respect to the torsional elasticity but not for unconfined axial compression. Microstructure analysis revealed impact of polymer type and concentration on dispersion quality.
dc.identifier.coursecodeKBTX61
dc.identifier.urihttp://hdl.handle.net/20.500.12380/306523
dc.language.isoeng
dc.setspec.uppsokPhysicsChemistryMaths
dc.subjectHydrogels
dc.subjectcrosslinking
dc.subjectosteoarthritis
dc.subjectcartilage
dc.subjectcell-therapy
dc.subjectrheology
dc.subjectmicroscopy
dc.titleHydrogels as scaffolds for stem cell therapy with focus on cartilage
dc.type.degreeExamensarbete för masterexamensv
dc.type.degreeMaster's Thesisen
dc.type.uppsokH
local.programmeBiotechnology (MPBIO), MSc
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