Bioprospecting for novel laminarin-degrading enzymes in marine microorganisms - A step towards the use of macroalgae in bioprocesses

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dc.contributor.authorOlsson, Joakim
dc.contributor.departmentChalmers tekniska högskola / Institutionen för biologi och biotekniksv
dc.contributor.departmentChalmers University of Technology / Department of Biology and Biological Engineeringen
dc.date.accessioned2019-07-03T13:48:59Z
dc.date.available2019-07-03T13:48:59Z
dc.date.issued2015
dc.description.abstractCurrently, efforts are being made to find alternative biomasses for biofuel production, which are not competing with food production. Several kelp species have potential for large-scale cultivation, harvest and biorefinery processes. On the Swedish west coast, Laminaria digitata and Saccharina latissima are two such species. These kelps can contain up to 33% of the storage carbohydrate laminarin (dry weight), which can be hydrolysed to glucose and subsequently converted into bioethanol by for instance yeast. Enzymes able to digest the β-1,3 or β-1,6 bonds of the laminarin, to release glucose, are in large unknown and this project has been about finding microorganisms expressing such enzymes, known as laminarases. Samples from partly decomposed L. digitata and S. latissima specimens were streaked on nutrient agar plates for proliferation and isolation of surface microorganisms. Isolated organisms were subsequently screened for growth on laminarin and promising strains were identified through 16s/18s rRNA sequencing. After growth experiments on liquid medium with different carbon sources, two bacterial strains, Pseudoalteromonas ssp., were selected for further characterisation. The two strains were grown in algae extract and the sugar composition was monitored over time. In the extract, mainly mannitol and laminarin were present but glucose was formed during the cultivation, indicating the presence of hydrolytic enzymes, after which the hydrolytic activity on pure laminarin was investigated. The supernatants of one of the strains showed in vitro activity, further indicating the presence of extracellular hydrolytic enzymes. More investigations are needed on whether the enzymes can be used in any type of process. This thesis work has been successful in isolating marine organisms with laminarin-degrading activity.
dc.identifier.urihttps://hdl.handle.net/20.500.12380/223273
dc.language.isoeng
dc.setspec.uppsokLifeEarthScience
dc.subjectEnergi
dc.subjectLivsvetenskaper
dc.subjectMiljöbioteknik
dc.subjectIndustriell bioteknik
dc.subjectBioprocessteknik
dc.subjectFytokemi inklusive alger och industribioråvaror
dc.subjectBiomaterial
dc.subjectEnergy
dc.subjectLife Science
dc.subjectEnvironmental Biotechnology
dc.subjectIndustrial Biotechnology
dc.subjectBioprocess Technology
dc.subjectPhytochemistry including algae and industrial bio-raw materials
dc.subjectBio Materials
dc.titleBioprospecting for novel laminarin-degrading enzymes in marine microorganisms - A step towards the use of macroalgae in bioprocesses
dc.type.degreeExamensarbete för masterexamensv
dc.type.degreeMaster Thesisen
dc.type.uppsokH
local.programmeBiotechnology (MPBIO), MSc
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