Developing data analysis methods for Nanofluidic Scattering Microscopy
Typ
Examensarbete för masterexamen
Master's Thesis
Master's Thesis
Program
Nanotechnology (MPNAT), MSc
Publicerad
2024
Författare
DOMENZAIN DEL CASTILLO CERECER, AARÓN
Modellbyggare
Tidskriftstitel
ISSN
Volymtitel
Utgivare
Sammanfattning
Nanofluidic Scattering Microscopy (NSM) is a label-free technique that allows the
simultaneous measurement of molecular weight and size of single molecules and
nanoparticles flowing through a nanochannel. Assuming free diffusion, the diffusivity
coefficient derived from the trajectory of a particle can be utilized to determine
its hydrodynamic radius via the Stokes-Einstein relation. However, non-specific
atractive interactions between biological nanoparticles and the inner walls of the
nanochannel, known as biofouling, immobilize the particle, invalidating the assumption
of free diffusion and making impossible to accurately determine the particle
size. This study introduces a computational method to detect biofouling in NSM,
based on the statistical analysis of instantaneous kinetic energy in discrete Brownian
motion. The method sets a probability threshold to either accept or reject the
hypothesis of free diffusion, allowing for the exclusion of biofouled segments from
diffusivity calculations, thereby enhancing the accuracy of hydrodynamic radius determination.
Beskrivning
Ämne/nyckelord
Nanofluidic scattering microscopy, biofouling, Brownian motion, hydrodynamic radius, computational algorithm