Protein binding determination of DHA in human plasma by HPLC using post-column on-line alkali derivatization and UV detection

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dc.contributor.authorZhang, Xiaonan
dc.contributor.departmentChalmers tekniska högskola / Institutionen för kemi- och biotekniksv
dc.contributor.departmentChalmers University of Technology / Department of Chemical and Biological Engineeringen
dc.date.accessioned2019-07-03T12:23:26Z
dc.date.available2019-07-03T12:23:26Z
dc.date.issued2010
dc.description.abstractArtemisinin and its derivatives are considered as a very important new class of antimalarials and becoming more and more commonly used throughout the world. Dihydroartemisinin (DHA) is the main bioactive metabolite of artemisinin in clinical use and has greater intrinsic atimalarial activity. Although pharmacokinetic research in vivo and in vitro has been done and conventional pharacokinetic parameters for DHA are well documented, data relating to parameter of protein binding of DHA is still inconsistent. In this project, equilibrium dialysis and ultrafiltration methods were carried out to determine the protein binding percentage of DHA in healthy human plasma. At the same time, HPLC conditions for DHA quantification wer optimized during experiments. Protein binding fraction of DHA was reported here as 80%-84% considering the volume shifts in equilibrium dialysis and 88%-91% in various DHA concentrations in ultrafiltration
dc.identifier.urihttps://hdl.handle.net/20.500.12380/126728
dc.language.isoeng
dc.setspec.uppsokPhysicsChemistryMaths
dc.subjectIndustriell bioteknik
dc.subjectIndustrial Biotechnology
dc.titleProtein binding determination of DHA in human plasma by HPLC using post-column on-line alkali derivatization and UV detection
dc.type.degreeExamensarbete för masterexamensv
dc.type.degreeMaster Thesisen
dc.type.uppsokH
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