Geometrical Axon Guidance with PDMS Microstructures

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dc.contributor.authorSpeicher, Sandra Ines
dc.contributor.departmentChalmers tekniska högskola / Institutionen för fysik (Chalmers)sv
dc.contributor.departmentChalmers University of Technology / Department of Physics (Chalmers)en
dc.date.accessioned2019-07-03T14:16:33Z
dc.date.available2019-07-03T14:16:33Z
dc.date.issued2016
dc.description.abstractHaving circuits made of single neurons would bring us a big step closer to a full understanding of the neuronal system. To build these networks and more, we need to find a way to guide axons in specific directions. Learning about their turning behavior in predefined geometric environments was the scope of this project. In order to enable a statistical analysis of the axons’ behaviors in geometrically defined environments, microstructures with repetitive patterns of T-junction channels were designed in AutoCAD and fabricated in polydimethylsiloxane (PDMS) from SU8 molds that had been made using photolithography. The design of the T-junctions was varied in different parameters such as the angle of incident of the upstroke of the ’T’ against the horizontal stroke or the relocation of the upstroke closer towards one side of the horizontal stroke. These structures were stuck onto laminin or poly-D-lysine (PDL) coatedWillCo dishes and seeded with primary cortical rat neurons. The neurons were transfected with a viral vector to enable fluorescence imaging with a confocal laser scanning microscope (CLSM).The time frame of the project did not allow for the performance of a statistical analysis, but important milestones were set for the preparation thereof. Structure designs that aid an automatized imaging process were found, the fragile culturing conditions primary neurons need to stay alive and grow axons were met and the automatization of the imaging itself was successfully implemented. A trend in the axon bending behavior was recognized.
dc.identifier.urihttps://hdl.handle.net/20.500.12380/239159
dc.language.isoeng
dc.setspec.uppsokPhysicsChemistryMaths
dc.subjectInformations- och kommunikationsteknik
dc.subjectMaterialvetenskap
dc.subjectGrundläggande vetenskaper
dc.subjectHållbar utveckling
dc.subjectInnovation och entreprenörskap (nyttiggörande)
dc.subjectFysik
dc.subjectInformation & Communication Technology
dc.subjectMaterials Science
dc.subjectBasic Sciences
dc.subjectSustainable Development
dc.subjectInnovation & Entrepreneurship
dc.subjectPhysical Sciences
dc.titleGeometrical Axon Guidance with PDMS Microstructures
dc.type.degreeExamensarbete för masterexamensv
dc.type.degreeMaster Thesisen
dc.type.uppsokH
local.programmeApplied physics (MPAPP), MSc
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