High Content Screening to Study Cellular Response to Treatment by Using Primary In Vitro Cultures Established from CNS Tumour Samples

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dc.contributor.authorEngdahl, Linus
dc.contributor.departmentChalmers tekniska högskola / Institutionen för biologi och biotekniksv
dc.contributor.examinerPetranovic Nielsen, Dina
dc.date.accessioned2020-02-20T15:37:26Z
dc.date.available2020-02-20T15:37:26Z
dc.date.issued2019sv
dc.date.submitted2019
dc.description.abstractThis study investigated if in vitro cultures of cancer stem cells derived from paediatric high-grade glioma tumours can be used to study the efficiency of therapeutic drugs by using custom analysis sequences with the Harmony software developed by Operetta. Four different drugs - vinorelbine, vincristine, etoposide, and methotrexate –, with and without irradiation, were tested. The nuclei were divided into subpopulations based on morphological and intensity properties with the hypothesis that each subpopulation corresponded to a specific cell state. The study also tested the theory that a subpopulation composed of presumably dead cells could be identified and used to discern drug effects not detected by studying the viable cell population alone. We found that each subpopulation demonstrated unique responses to the different treatments in concurrence with different cell states, showing that this method of subtyping has potential uses. It also demonstrates the possibility to use the subpopulation of dead cells in order to detect drug efficacy not readily apparent from observation of the total number of cells.sv
dc.identifier.coursecodeBBTX03sv
dc.identifier.urihttps://hdl.handle.net/20.500.12380/300703
dc.language.isoengsv
dc.setspec.uppsokLifeEarthScience
dc.subjectpaediatric cancersv
dc.subjectCNSsv
dc.subjectcancer stem cellssv
dc.subjectgliomasv
dc.subjectglioblastomasv
dc.subjecttreatmentsv
dc.subjectsubtypingsv
dc.subjectimage analysissv
dc.titleHigh Content Screening to Study Cellular Response to Treatment by Using Primary In Vitro Cultures Established from CNS Tumour Samplessv
dc.type.degreeExamensarbete för masterexamensv
dc.type.uppsokH
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