Exploring the Role of the ATRX Gene in High-Risk Neuroblastoma via Genome Engineering Techniques

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dc.contributor.authorJafargholizadeh, Setareh
dc.contributor.departmentChalmers tekniska högskola / Institutionen för life sciencessv
dc.contributor.departmentChalmers University of Technology / Department of Life Sciencesen
dc.contributor.examinerNygård, Yvonne
dc.contributor.supervisorMondal, Tanmoy
dc.date.accessioned2024-05-14T14:04:13Z
dc.date.available2024-05-14T14:04:13Z
dc.date.issued2023
dc.date.submitted
dc.description.abstractNeuroblastoma (NB) is a rare but aggressive pediatric cancer originating form the embryonic neural crest cells. Abnormal telomere maintenance has been implicated as a major cause of high-risk neuroblastoma, with a subset of tumors following the Alternative Lengthening of Telomere positive (ALT+) pathway. ALT+ tumors are difficult to treat due to their resistance to therapy. The exact cause of NB remains unclear, but recent research suggests genetic mutations as a possible cause, particularly mutations in the ATRX gene observed in ALT+ NB patients. This study focuses on understanding the role of the ATRX gene, in promoting ALT+ neuroblastoma. To investigate this, a dual approach was utilized, involving CRISPR/Cas9 technology for complete ATRX knockout and lentiviral shRNA silencing to reduce ATRX expression. The gene expression of ATRX has been successfully downregulated, but the successful knockout of ATRX could not be verified using CRISPR/Cas9 methods. Hence, additional validation is necessary to assess the impact of ATRX expression on ALT+ and to develop more effective strategies for addressing this subgroup of high-risk neuroblastoma (NB).
dc.identifier.coursecodeBBTX03
dc.identifier.urihttp://hdl.handle.net/20.500.12380/307685
dc.language.isoeng
dc.setspec.uppsokLifeEarthScience
dc.subjectNeuroblastoma
dc.subjectLentiviral vector
dc.subjectATRX
dc.subjectCRISPR/Cas9
dc.titleExploring the Role of the ATRX Gene in High-Risk Neuroblastoma via Genome Engineering Techniques
dc.type.degreeExamensarbete för masterexamensv
dc.type.degreeMaster's Thesisen
dc.type.uppsokH
local.programmeBiotechnology (MPBIO), MSc
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